魚類基因工程
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??學(xué)科組長：?胡煒??
學(xué)科組成員：朱作言研究員、院士；胡煒研究員；陳戟助理研究員?；研究生：陶彬彬，胡紅玲，馮科，彭偉等?
學(xué)科組介紹：魚類基因工程學(xué)科組的研究工作發(fā)端于上世紀(jì)70年代末的魚類細(xì)胞核移植研究。朱作言先生在著名實(shí)驗(yàn)胚胎學(xué)家童第周先生的領(lǐng)導(dǎo)下，通過合作研究完成鯉鯽間的細(xì)胞核移植，首次實(shí)現(xiàn)脊椎動物的種間克隆。上世紀(jì)80年代，朱作言先生在世界上首次成功進(jìn)行農(nóng)藝性狀轉(zhuǎn)基因研究，研制出世界首批轉(zhuǎn)基因魚，提出了轉(zhuǎn)基因魚形成的模型理論，開創(chuàng)魚類基因轉(zhuǎn)移研究新領(lǐng)域。以上成果作為近代中國科技領(lǐng)域的兩大重要科學(xué)技術(shù)成就被載入《世界科學(xué)年史》（A. Hellemans & B. Bunch, 美國Simon & Schuster出版社）。?
近10年來，本學(xué)科組合作培育出具有完全自主知識產(chǎn)權(quán)、生長速度快、餌料轉(zhuǎn)化率高的冠鯉和吉鯉；按照國家I類新藥的毒理學(xué)實(shí)驗(yàn)規(guī)范和實(shí)質(zhì)等同性原則，合作進(jìn)行了冠（吉）鯉的營養(yǎng)學(xué)、毒理學(xué)和致敏性研究，系統(tǒng)評價(jià)并證實(shí)冠（吉）鯉與對照鯉具有同樣的食用安全性；創(chuàng)建了“人工模擬湖泊”的生態(tài)安全實(shí)驗(yàn)體系，客觀評價(jià)了冠鯉投放到該人工湖泊后對生態(tài)環(huán)境的可能影響，發(fā)現(xiàn)在鯉魚自然分布的水域，冠鯉的生態(tài)風(fēng)險(xiǎn)不會高于對照鯉；為冠（吉）鯉的產(chǎn)業(yè)化準(zhǔn)入提供了充分的科學(xué)和材料儲備。?
此外，本學(xué)科組發(fā)現(xiàn)了魚類生長軸的信號分子在生殖發(fā)育中的作用、生殖軸/生長軸間新的調(diào)控通路及其作用機(jī)制；揭示了魚類生殖軸中重要信號分子調(diào)控性腺發(fā)育的機(jī)理；發(fā)現(xiàn)影響魚類配子減數(shù)分裂的信號分子在精子發(fā)育和成熟中的功能。首次報(bào)道了低溶氧耐受魚的成功研制。提出了魚類生殖操作育種新思路，創(chuàng)建了魚類育性可控的生殖開關(guān)新策略。?
目前，本學(xué)科組致力于經(jīng)濟(jì)魚類的分子雜交（轉(zhuǎn)基因）和分子突變（基因編輯）育種，以斑馬魚、青鳉等為模式系統(tǒng)的發(fā)育生物學(xué)研究。具體研究方向包括：魚類重要經(jīng)濟(jì)性狀相關(guān)基因或分子模塊的發(fā)掘與功能驗(yàn)證；魚類生殖發(fā)育、性別決定與分化機(jī)制解析；魚類生殖軸與生長軸之間的調(diào)控關(guān)系探索；魚類生殖開關(guān)策略的技術(shù)創(chuàng)新。?
以上研究得到了國家自然科學(xué)基金、中國科學(xué)院戰(zhàn)略性先導(dǎo)科技專項(xiàng)和湖北省自然科學(xué)基金創(chuàng)新群體基金以及科技部863計(jì)劃和973計(jì)劃等項(xiàng)目的資助。?
近年論文發(fā)表目錄：?
2016年?
1.??Chen Ji. Cui Xiaojuan, Jia Shaoting, Luo Daji, Cao Mengxi, Zhang Yunsheng, Hu Hongling, Huang Kaiyao, Zhu Zuoyan & Hu Wei.?Disruption of?dmc1?Produces Abnormal Sperm in Medaka (Oryzias latipes). Scientific Reports,?2016, 6, 30912; doi: 10.1038/srep30912.?
2.??Mikwar M, Navarro-Martin L, Volkoff H, Hu Wei and Trudeau V.L. Stimulatory effect of the secretogranin-ll derived peptide secretoneurin on food intake and locomotion in female goldfish?(Carassius auratus), Peptides, 2016 Apr;78:42-50. ?
3.??Peng Wei, Cao Mengxi, Chen Ji, Li Yongming, Wang Yaping, Zhu Zuoyan, Hu Wei, GnIH plays a negative role in regulating GtH expression in the common carp,?Cyprinus carpio?L. General and Comparative Endocrinology, 2016, 235 :18–28.?
4.??Shu Yuqin, Lou Qiyong, Dai Ziru, Dai Xiangyan, He Jiangyan, Hu Wei & Yin Zhan, The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models. Scientific Reports, 2016, 6:18597, DOI: 10.1038/srep18597.?
5.??Zhou Y, Wang M, Jiang M, Peng L, Wan C, Liu J, Liu W, Zhao R, Zhao X, Hu W, Liu S, Xiao Y. Autotetraploid cell line induced by SP600125 from crucian carp and its developmental potentiality. Scientific Reports, 2016, 6:21814. doi: 10.1038/srep21814.?
6.??Zhu T. B, Zhang L. H, Zhang T. L, Wang Y. P, ?Hu W, ?Ring？ E.and Zhu Z. Y.?.Effects of sustained predation by fast-growing transgenic common carp (Cyprinus carpio?Linnaeus, 1758) on gastropods in artificial environments.?J. Appl. Ichthyol.?2016; DOI:?10.1111/jai.13130?
7.??胡煒，朱作言。美國轉(zhuǎn)基因大西洋鮭產(chǎn)業(yè)化對我國的啟示。中國工程科學(xué)， 2016，18（3）：105-109。?
2015年?
1.???Chen Ji, Luo Qing, Bao Hairong, Liao Lanjie, Li Yongming, Zhu Zuoyan, Wang Yaping, Hu Wei.?The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio?L.) with fast-growth performance. Science Bulletin, 2015, 60(19):1654–1660。?
2.???Chen Rong, Qi Jianxun, Yuan Hongyu, WuYanan, Hu Wei, Xia Chun. Crystal structures for short-chain pentraxin from zebrafish demonstrate a cyclic trimer with new recognition and effector faces. Journal of Structural Biology 189 (2015) 259–268.?
3.???Hu Wei and Chen Ji. Whole-genome sequencing opens a new era for molecular breeding of grass carp (Ctenopharyngodon idellus). Science China, life Sciences, 2015, 58(6): 619–620.?
4.???Luo Daji, Liu Yun, Chen Ji, Xia Xiaoqin, Cao Mengxi, Cheng Bin, Wang Xuejuan, Gong Wuming, Qiu Chao, Zhang Yunsheng, Cheng Christopher Hon Ki, Zhu Zuoyan & Hu Wei. Direct production of XYDMY？sex reversal female medaka (Oryzias latipes) by embryo microinjection of TALENs. Scientific Reports,5:14057, DOI: 10.1038/srep14057?
5.???Song Yanlong, DuanXiaohai, Chen Ji, Zhu Zuoyan, Hu Wei. The distribution of Kiss1- and Kiss2-positive neurones and their connections with GnRH3 neurones in the zebrafish brain. Journal of Neuroendocrinology, 2015, 27(3): 198–211.?
6.???Tang Haipei, Liu Yun, Luo Daji, Ogawa Satoshi, Yin Yike, Li Shuisheng, Zhang Yong, Hu Wei, Parhar Ishwar S, Lin Haoran, Liu Xiaochun, Cheng Christopher H.K.. The kiss/kissr systems are dispensable for zebrafish reproduction: evidence from gene knockout studies，Endocrinology, 2015, 156:589-599.?
7.???Wang Y, Lu Y, Zhang Y, Ning Z, Li Y, Zhao Q, Lu H, Huang R, Xia X, Feng Q, Liang X, Liu K, Zhang L, Lu T, Huang T, Fan D, Weng Q, Zhu C, Lu Y, Li W, Wen Z, Zhou C, Tian Q, Kang X, Shi M, Zhang W, Jang S, Du F, He S, Liao L, Li Y, Gui B, He H, Ning Z, Yang C, He L, Luo L, Yang R, Luo Q, Liu X, Li S, Huang W, Xiao L, Lin H, Han B, Zhu Z. The draft genome of the grass carp (Ctenopharyngodon idellus) provides insights into its evolution and vegetarian adaptation. Nature Genetics. 2015; 47(6): 625-631.?
8.???Zhang Yunsheng, Chen Ji, Cui Xiaojuan, Luo Daji, Xia Hui, Dai Jun, Zhu Zuoyan, Hu Wei. A controllable on-off strategy for the reproductive containment of fish. Scientific Reports, 2015, 5, 7614; DOI:10.1038/srep07614.?
2014年?
1.???Cao Mengxi, Chen Ji, Peng Wei, Wang Yaping, Liao Lanjie, Li Yongming, Trudeau Vance L., Zhu Zuoyan, Hu Wei. Effects of growth hormone over-expression on reproduction in the common carp?Cyprinus carpio?L. General and Comparative Endocrinology, 2014,195:47-57.?
2.???Chu Lianhe, Li Jianzhen, Liu Yun, Hu Wei, Cheng Christopher H.K.. Targeted gene disruption in zebrafish reveals noncanonical functions of Lh signaling in reproduction.Molecular Endocrinology, 2014, 28(11):1785-95.?
3.???Garcia-ReyeroNatàlia, Tingaud-SequeiraAngèle, Cao Mengxi, Zhu Zuoyan, Perkins Edward, Hu Wei. Endocrinology: Advances through omics and related technologies, General and Comparative Endocrinology, 2014, 203:262-273.?
4.???Liu Y, Luo D, Lei Y, Zhao H, Hu W, Cheng HK, A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish, Methods, 2014, 69: 58–66.?
5.???QiuChao, Cheng Bin, Zhang Yunsheng, Huang Rong, Liao Lanjie, Luo Daji, Hu Wei, Wang Yaping. Efficient Knockout of Transplanted Green Fluorescent Protein Gene in Medaka using TALENs. Marine Biotechnology. 2014, 16(6):674-83?
6.?? Zhang L., Gozlan R. E., Li Z., Liu J., Zhang T., Hu W and Zhu Z. Rapid growth increases intrinsic predation riskin genetically modified?Cyprinus carpio: implications for environmental risk. Journal of Fish Biology，2014，84：1527–1538.?
2013年?
1.???Chen Ji, Hu Wei & Zhu ZuoYan. Progress in studies of fish reproductive development regulation. Chinese Science Bulletin, 2013,58:7-16.?
2.???Duan M., Zhang T., Hu W., Xie S., Sundstr¨om L. F, Li Z. and Zhu Z. Risk-taking behaviour may explain high predation mortality of GH-transgenic common carp?Cyprinus carpio. Journal of Fish Biology, 2013, 83: 1183-1196?
3.???Li X, Yan Q, Xie S, Hu W, Yu Y, Hu Z. Gut Microbiota Contributes to the Growth of Fast-Growing Transgenic Common Carp (Cyprinus carpio?L.). Plos one, 2013, 8(5): e64577. ?
4.???Lian Hao, Hu Wei, Huang Rong, Du Fukuan, Liao Lanjie, Zhu Zuoyan, Wang Yaping. Transgenic Common Carp Do Not Have the Ability to Expand Populations. Plos one, 2013，8(6): e65506.?
5.?? Liu Yun, Luo Daji, Zhao Hui, Zhu Zuoyan, Hu Wei, Cheng Christopher H. K.. Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in Zebrafish Using TALENs. PLoS ONE, 2013, 8(10): e76387.?
6.???Zhong C, Song Y, Wang Y, Zhang T, Duan M, Li Y, Liao L, Zhu Z, Hu W. Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio?L.) may be mediated by upregulating Agouti-related protein (AgRP). General and Comparative Endocrinology，2013，192, 81-88.?
7.???Zhu T, Zhang T, Wang Y, Chen Y, Hu W, Zhu Z. Effects of growth hormone (GH) transgene and nutrition on growth and bone development in common carp. J. Exp. Zool. 2013, 319A:451-460.?
8.???陳戟，羅余山，朱作言，胡煒, 斜帶石斑魚（Epinephelus coioides）GnRHⅡ基因的克隆及啟動子分析. 遺傳，2013, 35(7): 896-902.?
2012年?
1.???Cao Mengxi, Duan Jundan, Cheng Nana, Zhong Xueping, Wang Zequn, Hu Wei, Zhao Haobin. Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in?Gobiocypris rarus. Comparative Biochemistry and Physiology, Part A, 2012, 162: 303–309.?
2.??Cao Mengxi, Yang Yuhui, Xu Hongyan, Duan Jundan, Cheng Nana, Wang Jialin, Hu Wei, Zhao Haobin. Germ cell specific expression of Vasa in rare minnow,?Gobiocypris rarus,?Comparative Biochemistry and Physiology, Part A, 2012, 162:163–170.?
3.??Yang Yuhui, Peng Kou, Liu Xiaoyan, Zhao Dongmei, Duan Jundan, Hu Wei, Zhao Haobin. Effects of sex steroids on expression of myostatin in rare minnow,?Gobiocypris rarus. Aquaculture, 2012, 350-353: 1–7.?
4.??Zhong Chengrong, Song Yanlong, Wang Yaping, Li Yongming, Liao Lanjie, Xie Shouqi, Zhu Zuoyan, Hu Wei. Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio?L.). Aquaculture, 2012, 356–357: 404–411.?
2011年?
1.??Duan M, Zhang T L, Hu W, Li Z J, Sundstrom L F, Zhu T B, Zhong C R & Zhu Z Y. Behavioral alterations in GH transgenic common carp may explain enhanced competitive feeding ability. Aquaculture, 2011, 317: 175-181。?
2.??Feng Hong, Liu Hong, Kong Renqiu, Wang Lu, Wang Yaping, Hu Wei, Guo Qionglin. Expression profiles of carp IRF-3/-7 correlate with the up-regulation of RIG-I/MAVS/TRAF3/TBK1, four pivotal molecules in RIG-I signaling pathway. Fish & Shellfish Immunology 2011: 30(4-5): 1159-1169.?
3.??Guan Bo, Ma Hong, Wang Yaping, Hu Yuanlei, Lin Zhongping, Zhu Zuoyan, Hu Wei. Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio). Marine Biotechnology, 2011, 13（2）: 336-344. (cover paper)?
4.??Li Deliang, FU Cuizhang, Wang Yaping, Zhu Zuoyan, Hu Wei. The hematological response to exhaustive exercise in ‘a(chǎn)ll-fish’ growth hormone transgenic common carp (Cyprinus carpio?L.). Aquaculture, 2011,311:263-268.?
5.??Luo Daji, Hu Wei, Chen Shangping, Zhu Zuoyan. Critical Developmental Stages For The Efficiency Of Somatic Cell Nuclear Transfer In Zebrafish. International Journal of Biological Sciences, 2011,7: 476-486. ?
6.??Xu Jing, Huang Wei, Zhong Chengrong, Luo Daji, Li Shuangfei, Zhu Zuoyan and Hu Wei, Defining Global Gene Expression Changes of the Hypothalamic-Pituitary-Gonadal Axis in Female sGnRH-Antisense Transgenic Common Carp (Cyprinus carpio), PLoS One, 2011, 6(6): e21057. ?
7.??Yu Fan, Xiao Jun, Liang XiangYang, Liu ShaoJun, Zhou GongJian, Luo KaiKun, Liu Yun, Hu Wei, Wang YaPing & Zhu ZuoYan. Rapid growth and sterility of growth hormone gene transgenic triploid carp. Chinese Science Bulletin: 2011, 56(16): 1679-1684.?
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發(fā)明專利目錄?
1.?Hu Wei, Zhang Yunsheng, Dai Jun and Zhu Zuoyan. Controllable on-off method for fish reproduction. 美國發(fā)明專利，2015，專利號：US 9,187,764 B2?
2.?Hu Wei, Zhu Z, Ma H, Guan B, Lin Z, and Hu R. Recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen stress and the use thereof. 美國發(fā)明專利，2013，專利號: US8546644 B2。?
3.?Hu Wei, Zhu Zuoyan, Wang Yaping and Li Shuangfei. Method for preparing a sterile transgenic carp. 美國發(fā)明專利，2007，專利號：US7,230,156B2。?
4.?胡煒，馮科，陳戟，朱作言。一種黃鱔基因編輯的方法。中國發(fā)明專利，2016，申請?zhí)枺?01610651812.1?
5.?胡煒，張運(yùn)生，戴軍，朱作言。一種控制魚類生殖的方法。中國發(fā)明專利，2014，專利號：ZL201310049161.5。?
6.?胡煒，湯斌，汪亞平，朱作言，一種雌性鯉魚性逆轉(zhuǎn)為雄性鯉魚的培育方法，中國發(fā)明專利，2012，專利號： ZL201110186519.X。?
7.?胡煒，朱作言，汪亞平，黎雙飛。不育轉(zhuǎn)基因魚的制備方法，中國發(fā)明專利，2007，專利號：ZL031254551。?
8.?胡煒，朱作言，黎雙飛，汪亞平。一種殼聚糖甲基睪酮緩釋藥條的制備方法，中國發(fā)明專利，2007，專利號：ZL200510018148.9。?
9.?吳玉萍，朱作言，胡煒，徐安龍。魚類基因原位修飾育種的方法，中國發(fā)明專利授權(quán)，2006，專利號：ZL200410015247.7?
10. 朱作言，鐘家玉。一種魚類電脈沖精子轉(zhuǎn)基因方法，中國發(fā)明專利，2004，專利號：ZL01114202.2?
