魚類發(fā)育與干細胞
? 學(xué)科組成員：孫玉華?研究員；助理研究員孫曉云，在讀博士4人，在讀碩士2人。??
? 學(xué)科組介紹：?
本學(xué)科組關(guān)注基礎(chǔ)研究和應(yīng)用基礎(chǔ)研究。一方面研究生命科學(xué)的基本問題，即受精卵如何發(fā)育成為一個完整的個體？另一方面，圍繞基礎(chǔ)科研服務(wù)于國家需求，從干細胞理論和技術(shù)出發(fā)，研究重大疾病的發(fā)病機理、開展干細胞治療研究。本課題組以斑馬魚和哺乳動物多能干細胞為材料，在以下方向開展研究：脊椎動物早期胚胎發(fā)育、多能干細胞自我更新和分化機理、干細胞治療和轉(zhuǎn)化醫(yī)學(xué)等。主要研究內(nèi)容如下：?
1、以斑馬魚為模式生物，研究轉(zhuǎn)錄因子、信號通路、表觀遺傳因子以及染色質(zhì)高級結(jié)構(gòu)等調(diào)控脊椎動物早期胚胎發(fā)育、細胞命運決定以及基因表達的分子機制。為治療人類發(fā)育疾病提供機理解釋。?
2、以人和小鼠多能干細胞為材料，揭示干細胞自我更新與維持、分化的細胞及分子生物學(xué)基礎(chǔ)。鑒定調(diào)控干細胞自我更新和分化的關(guān)鍵因子；闡明其在干細胞自我更新和分化、細胞命運決定中的作用機制。?
3、干細胞治療和轉(zhuǎn)化醫(yī)學(xué)研究。研究內(nèi)容包括：干細胞理論和技術(shù)研究重大疾病發(fā)病機理；基于多能干細胞的體外類器官（類腸、類心臟等）構(gòu)建，闡明其定向分化及自組裝規(guī)律；干細胞和組織工程結(jié)合研發(fā)新型臨床替代物等等。 ?
本課題組得到國家重點研發(fā)計劃項目資助，總研究經(jīng)費約600萬元。?
???近年來發(fā)表文章：
Sun, X., Peng, X., Cao, Y., Zhou, Y., and?Sun, Y. (2020). ADNP promotes neural differentiation by modulating Wnt/beta-catenin signaling.?Nat Commun?11, 2984.?
Sun, X.Y.; Yu, W.J.; Li, L.;?Sun, Y.H.?(2020): ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4. Frontiers in?cell and developmental biology?.?
?? Sun, X.Y., Chen, J., Zhang, Y.Y., Munisha, M., Dougan, S., and?Sun, Y.H.?(2018). Mga Modulates Bmpr1a Activity by Antagonizing Bs69 in Zebrafish.?Frontiers in cell and developmental biology?6.??
Ryan Berger,?Yuhua Sun?et al., (2016). ST8SIA4-dependent polysialylation is part of a developmental program required for germ layer formation from human pluripotent stem cells.?Stem Cells,?DOI:?10.1002/stem.2379? ??
Amar Singh*,?Yuhua Sun*, Li Li, Steve Qin, and Steve Dalton (2015). Cell-Cycle ?Control of Bivalent Epigenetic Domains Regulates the Exit from Pluripotency.?Stem?Cell?Reports，3:323 (*equal contribution)? ??
Yuhua Sun, Weichia Tseng, Rebecca Ball,and Scott Dougan (2014). Extra-embryonic signals under the control of Mga, Max and Smad4 are required for dorsoventral patterning.?Developmental Cell, 28: 322? ??
J Champell*, YH Sun*,?A Singh, S Dalton (2013).?Myc/Max control Erk signaling and pluripotency by regulation of dual specificity phosphatases 2 and 7.?Genes & Development (*equal contribution),?27:725-733? ??
J Phillips-Cremins, M Sauria, A Sanyal, T Gerasimova, B Lajoie, J Bell, CT Ong, T Hookway, C Guo,?YH Sun, M Bland, W Wagstaff, S Dalton, T McDevitt, R Sen, J Dekker, J Taylor, V Corces (2013)．Architectural protein subclasses shape 3- D organization of genomes during lineage commitment．Cell, 153: 1281? ??
Amar M. Singh, David Reynoids, Timothy Cliff, Satoshi Ohtsuka, Alexa Mattheyses,?Yuhua Sun,?Laura Menendez, Michael Kulik and Stephen Dalton (2012).?Signaling Network Crosstalk in Human Pluripotent Cells: A Smad2/3-Regulated Switch that Controls the Balance between Self-Renewal and Differentiation.?Cell stem cell, 10(3): 312-326? ??
Sun YH, Wloga D. and Dougan S.?(2011).?Embryological manipulations in zebrafish.?Methods Mol. Biol., 770: 139-184? ??
D.M. Webster, C.F. Teo,?YH. Sun, D. Wloga, S. Gay, L. Wells and S. T. Dougan (2009). O-GlcNAc modifications regulate cell survival and epiboly during zebrafish development.?BMC Dev. Biol., 9(1): 28-33?Highly Accessed Article? ??
Chengtai Yang, Ling Cao, Weimin Wang, Yi Yang, Binlun Yan, Li Su,?Yuhua Sun, and Huanling Wang (2009).?Comparative and evolutionary analysis in natural diploid and tetraploid weather loach Misgurnus anguillicaudatus based on cytochrome b sequence data in central China.?Environmental Biology of Fishes, 86(1): 145-153? ??
Treer T., Sprem N, Torcu-Koc,?YH Sun, and M. Piria (2008).?Length-weight relationship of freshwater fishes of Croatia.?J. Appl. Ichthyol, 24: 626-628? ??
Yuhua Sun,?Rong Tang, Dapeng Li, Xuezhen Zhang, Juan Fu, Ping Xie (2008).?Acute effects of microcystins on transcription of antioxidant enzyme genes in crucian carp.?Environ. Toxicol.,?23: 145-152?? ??
Zexia Gao, *Weimin Wang, Khalid Abbas, Xiaoyun Zhou, Yi Yang, James S Diana, Hanping Wang, Huanling Wang, Yang Li,?Yuhua Sun?(2007). Haematological characterization of loach Misgurnus anguillicaudatus: comparison among diploid, triploid and tetraploid specimens.?Comp Biochem Physiol A Mol Integr Physiol.?2007 Aug;147-? ??
YH Sun,?CX Xie, WM Wang, SY Liu, T. Treer, MM Chang et al (2007).?The genetic variation and the biogeography of catostomid fishes based on mitochondrial and nucleic DNA sequences.?Journal of Fish Biology, 70: 291-309?? ??
Yuhua Sun, Siyang Liu, Gang Zhao, QX Yu et al (2004).?Population structure of Chinese sucker Myxocyprinus asiatus (Cypriniformes: Catostomidae) in the Yangtze River based on Mitochondrial DNA control region sequences.?Fisheries Science, 70(3): 412-420? ??
SUN Yu-Hua;?WANG Wei; LIU Si-Yang; HE Shun-Ping; SHAO Xue-Ling; XIE Zhi-Xiong; DENG Feng-Jiao; LIU Yong; TONG Jin-Gou; WU Qing-Jiang (2002).?Genetic Diversity Analysis of Mitochondrial D-loop Region of Chinese Sucker (Myxocyprinus asiaticus). Journal of Genetics and Genomics (in Chinese),?29(9): 787-790 ?
