環(huán)境毒理學(xué)
學(xué)科帶頭人：韓建?項(xiàng)目研究員
學(xué)科組介紹：環(huán)境毒理組目前進(jìn)行的研究方向是持久性有機(jī)污染物 (POPs) 對(duì)生物作用的分子機(jī)制及生態(tài)毒理學(xué)效應(yīng)。研究主要在兩個(gè)方面進(jìn)行:
(1) 以培養(yǎng)的細(xì)胞株或原代細(xì)胞培養(yǎng)，用來(lái)研究污染物作用的分子機(jī)制。建立靈敏、高效的生物毒性測(cè)試方法，評(píng)價(jià)有機(jī)化學(xué)品的潛在毒性效應(yīng)。
(2) 研究有毒污染物在水環(huán)境中的遷移轉(zhuǎn)化過(guò)程及生態(tài)風(fēng)險(xiǎn)。以小型淡水魚(yú)作為活體模型研究分子機(jī)理及生態(tài)學(xué)效應(yīng)。.研究工作將微觀與宏觀相結(jié)合，從分子/生化/生理/個(gè)體層次上進(jìn)行，并建立之間的聯(lián)系，探索有機(jī)污染物對(duì)水生生物的生殖毒性、神經(jīng)毒性、發(fā)育毒性、健康危害，以及多種污染物在環(huán)境條件下的復(fù)合作用，尋找更敏感、有效的生物標(biāo)記物，發(fā)展環(huán)境污染的早期預(yù)警系統(tǒng)，從而為有機(jī)污染物的危險(xiǎn)評(píng)價(jià)以及為我國(guó)可持續(xù)發(fā)展戰(zhàn)略、生態(tài)安全及環(huán)境保護(hù)決策提供理論依據(jù)。
學(xué)科組現(xiàn)得到國(guó)家科技部863計(jì)劃、國(guó)家自然科學(xué)基金項(xiàng)目、中國(guó)科學(xué)院知識(shí)創(chuàng)新重要方向項(xiàng)目等項(xiàng)目的資助:
1. 典型持久性有機(jī)污染物的環(huán)境過(guò)程與毒理效應(yīng)：子課題“典型持久性有機(jī)污染物的毒理效應(yīng)”（國(guó)家自然科學(xué)基金重大項(xiàng)目）
2. 典型全氟代有機(jī)化合物的發(fā)育毒性及雌激素效應(yīng)的分子機(jī)理研究（國(guó)家自然科學(xué)基金面上項(xiàng)目）
3. 有毒污染物的分子毒理與篩選技術(shù)子課題：離體細(xì)胞系的構(gòu)建和有毒污染物離體篩選技術(shù)的研究（中科院知識(shí)創(chuàng)新重要方向項(xiàng)目）
4. 溴系阻燃劑暴露及效應(yīng)的評(píng)估新技術(shù)（國(guó)家863計(jì)劃）
5. 有機(jī)污染物全氟辛烷磺?；衔锏姆肿由鷳B(tài)毒理學(xué)效應(yīng)及危險(xiǎn)評(píng)價(jià) （自然科學(xué)基金面上項(xiàng)目）
6. 持久性有機(jī)污染物的分子生態(tài)毒學(xué)效應(yīng)及生物可降解性研究 （中國(guó)科學(xué)院知識(shí)創(chuàng)新重要方向項(xiàng)目）
7. 建立新的生物學(xué)模型以篩選和評(píng)價(jià)有機(jī)化合物的毒性及對(duì)水生態(tài)環(huán)境的影響
8. 環(huán)境污染物持續(xù)性作用的健康效應(yīng)及致病機(jī)制研究（湖北省科技攻關(guān)計(jì)劃）
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儀器設(shè)備：倒置熒光顯微鏡，紫外-可見(jiàn)光-熒光酶標(biāo)儀，超純水制備系統(tǒng)，細(xì)胞培養(yǎng)系列設(shè)備，PCR儀，凝膠成像，高速冷凍離心機(jī)，超低溫冰箱，雜交箱，切片機(jī)。
近三年發(fā)表文章：
1. Xiongjie Shi, Leo W. Y. Yeung, Paul K. S. Lam, Rudolf S. S. Wu,?Bingsheng Zhou*?(2009). Protein Profiles in Zebrafish (Danio rerio) Embryos Exposed to PFOS.?Toxicological Sciences?(in press).
2. Chunsheng Liu, Liqin Yu, Jun Deng, Paul K. S. Lam, Rudolf S. S. Wu,?Bingsheng Zhou*?(2009). Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic–pituitary–gonadal (HPG) axis of zebrafish.?Aquatic Toxicology?(in press).
3. Jun Deng, Liqin Yu, Chunsheng Liu, Ke Yu, Xiongjie Shi, Leo WY Yeung, Paul KS Lam, Rudolf SS Wu,?Bingsheng Zhou*(2009). HBCD-induced developmental toxicity and apoptosis in zebrafish embryos.?Aquatic Toxicology?(in press).?
4. 史熊杰，劉春生，余珂，鄧軍，余麗琴，周炳升*（2009）。環(huán)境內(nèi)分泌干擾物毒理學(xué)研究進(jìn)展與展望。化學(xué)進(jìn)展，21，340-349。
5. Yongbing Du, Xiongjie Shi, Chunsheng Liu, Ke Yu,?Bingsheng Zhou* (2009). Chronic effects of water-borne PFOS exposure on growth, survival and hepatotoxicity in zebrafish: A partial life-cycle test.?Chemosphere, 74, 723-729.
6. Ke Yu, Yuhe He, Leo W.Y. Yeung, Paul KS Lam, Rudolf SS Wu,?Bingsheng Zhou* (2008). DE-71-induced apoptosis involving intracellular calcium and the Bax-mitochondria-caspase protease pathway in human neuroblastoma cells in vitro.?Toxicological Sciences, 104, 341-351.
7. Xiongjie Shi, Yongbing Du, Paul KS Lam, Rudolf SS Wu,?Bingsheng Zhou* (2008). Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS.?Toxicology & Applied Pharmacology, 230, 23-32.?
8. Chunsheng Liu, Yongbing Du,?Bingsheng Zhou* (2007). Evaluation of estrogenic activities and mechanism of action of perfluorinated chemicals determined by vitellogenin induction in primary cultured tilapia hepatocytes.?Aquatic Toxicology, 85，267-277.?
9. So M.K., Y. Miyake, W.Y. Yeung, Y.M. Ho, S. Taniyasu, P. Rostkowski, N. Yamashita,?B.S. Zhou, X.J. Shi, J.X. Wang, J.P. Giesy, H. Yu and P.K.S. Lam* (2007). Perfluorinated compounds in the Pearl River and Yangtze River of China.?Chemosphere,?68, 2085-2095.
10. Wen Yi, Wang Ying,?Zhou Bing-Sheng, Xu Ying and Feng Yu-Qi* (2007). Determination of Sexual Hormones in Liquid Cosmetics by Polymer Monolith Microextraction Coupled with High Performance Liquid Chromatography.?Chinese Journal of analytical Chemistry, 35, 681-684.
11. Chunsheng Liu, Ke Yu, Xiongjie Shi, Jingxian Wang, Paul K.S. Lam, Rudolf S.S. Wu and?Bingsheng Zhou* (2007). Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus).?Aquatic Toxicology, 82:135-143.
12. Ling Ding, Margaret B. Murphy, Henry Y.H. He, Yan Xu, Leo W.Y. Yeung, Jingxian Wang,?Bingsheng Zhou*, Paul K.S. Lam, Rudolf S.S. Wu and John P. Giesy (2007). Effects of brominated flame retardants and brominated dioxins on steroidgenesis in H295R human adrenocortical carcinoma cell line.?Environmental Toxicology & Chemistry, 26: 764-772.
13. Yi Wen,?Bingsheng Zhou, Ying Xu, Shiwei Jin, Yuqi Feng* (2006). Analysis of estrogens in environmental waters using polymer monolith in polyether ether ketone tube solid-phase microextraction combined with high-performance liquid chromatograph.?Journal of Chromatography?A, 1133: 21-28.?
14. S.B. Song, Y. Xu* and??B.S. Zhou?(2006). Effects of hexachlorobenzene on antioxidant status of liver and brain of common carp (Cyprinus carpio).?Chemosphere, 65: 699-706.
15.?Bingsheng Zhou, Wenhua Liu, William H.L. Siu, Paul K.S. Lam and Rudolf S.S. Wu* (2006). Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress.?Aquatic Toxicology, 77: 136-142.
16.?Bingsheng Zhou*, Chunsheng Liu, Jingxian Wang, Paul K.S. Lam, Rudolf S.S. Wu (2006). Primary cultured cells as sensitive in vitro model for assessment of toxicants-comparison to hepatocytes and gill epithelia.?Aquatic Toxicology, 80, 109-118.
