原生動(dòng)物多樣性與生物技術(shù)研究組
研究組組長：繆煒?miaowei@ihb.ac.cn?
研究組成員：
熊杰 (研究員)?、龔迎春 (研究員)?
黃潔 (副研究員)、羅曉甜 (副研究員) 、姜傳奇 (副研究員)
袁冬霞 (實(shí)驗(yàn)師)、王光營（特別研究助理）、張晶（特別研究助理）、陳凱（特別研究助理）、程子昕（特別研究助理）、谷思雨（特別研究助理）、涂家薇（特別研究助理）
研究生16名，其中博士研究生6名，碩士研究生10名
學(xué)科組介紹：
傳承于享譽(yù)國內(nèi)外的原生動(dòng)物學(xué)家王家楫院士創(chuàng)建、沈韞芬院士發(fā)展的原生動(dòng)物分類與生態(tài)學(xué)學(xué)科組。以單細(xì)胞真核微生物——原生動(dòng)物為研究對象，綜合利用形態(tài)學(xué)、分子生物學(xué)、組學(xué)、生物信息學(xué)和實(shí)驗(yàn)生物學(xué)等多種手段，研究原生動(dòng)物的分類和生物系統(tǒng)學(xué)，建立關(guān)鍵種質(zhì)和遺傳資源；解析原生動(dòng)物的重要性狀和功能演化及生態(tài)適應(yīng)機(jī)制；發(fā)展原生動(dòng)物合成生物學(xué)模型和關(guān)鍵生物技術(shù)，開展原生動(dòng)物資源利用和應(yīng)用研究。? ??
近期主要研究方向：?
1）原生動(dòng)物多樣性研究：
長期廣泛開展野外調(diào)查，發(fā)展環(huán)境DNA和單細(xì)胞組學(xué)等關(guān)鍵技術(shù)，對原生動(dòng)物主要類群和自由生/寄生/共生代表種類進(jìn)行收集、鑒定和分類，建立原生動(dòng)物種質(zhì)、遺傳資源信息庫以及環(huán)境DNA數(shù)據(jù)庫；結(jié)合形態(tài)、組學(xué)及基因功能等多維數(shù)據(jù)，理解原生動(dòng)物系統(tǒng)發(fā)育關(guān)系，生物多樣性及其形成機(jī)制；發(fā)展纖毛蟲、鞭毛蟲、變形蟲等多種原生動(dòng)物研究模型，研究單細(xì)胞原生動(dòng)物適應(yīng)性進(jìn)化/生態(tài)適應(yīng)的功能基礎(chǔ)，深入探討生殖、運(yùn)動(dòng)、捕食和共生等重要基礎(chǔ)或進(jìn)化生物學(xué)問題。?
2）原生動(dòng)物生物技術(shù)：
發(fā)掘纖毛蟲、鞭毛蟲、變形蟲原生動(dòng)物中重要種質(zhì)和遺傳資源，發(fā)展以四膜蟲為主的單細(xì)胞原生動(dòng)物合成生物學(xué)模型，建立基因編輯、大片段DNA合成和組裝、細(xì)胞賦能等關(guān)鍵技術(shù)，研發(fā)用于環(huán)境污染物監(jiān)測/降解、有害藻類控制和農(nóng)業(yè)病害防治的新技術(shù)和新策略?！??
代表性論文與著作:?
????1.Yang Ma#, Guanxiong Yan#, Jing Zhang, Jie Xiong, Wei Miao*. Cip1, a CDK regulator determines heterothallic mating or homothallic selfing in a protist. PNAS, 2024, 121?(13):?e2315531121.
????2.Chuanqi Jiang,?Siyu Gu,?Tingting Pan,?Xueyan Wang,?Weiwei Qin,?Guangying Wang,?Xinxin Gao,?Jing Zhang,?Kai Chen,?Alan Warren,?Jie Xiong,?Wei Miao*. Dynamics and timing of diversification events of ciliated eukaryotes from a large phylogenomic perspective. Molecular Phylogenetics and Evolution, 2024, 197, 108110.
????3.Mingyue Cheng#, Shuai Luo#, Peng Zhang#, Guangzhou Xiong, Kai Chen, Chuanqi Jiang, Fangdian Yang, Hanhui Huang, Pengshuo Yang, Guanxi Liu, Yuhao Zhang, Sang Ba, Ping Yin, Jie Xiong*, Wei Miao*, Kang Ning*. A comprehensive genome and gene catalog of aquatic microbiome in Tibetan Plateau. Nature Communications, 2024, 15, 1438.
????4.Xinxin Gao#, Kai Chen#, Jie Xiong#, Dong Zou#, Fangdian Yang, Yingke Ma, Chuanqi Jiang, Xiaoxuan Gao, Guangying Wang, Siyu Gu, Peng Zhang, Shuai Luo, Kaiyao Huang, Yiming Bao, Zhang Zhang*, Lina Ma*, Wei Miao*. The P10K database: A data portal for the protist 10,000 genomes project. Nucleic Acids Research, 2024, 52(D1): D747-D755.
????5.Chuanqi Jiang#,?Guangying Wang#,?Jing Zhang,?Siyu Gu,?Xueyan Wang,?Weiwei Qin,?Kai Chen,?Dongxia Yuan,?Xiaocui Chai,?Mingkun Yang,?Fang Zhou,?Jie Xiong*,?Wei Miao*. iGDP: an integrated Genome Decontamination Pipeline for wild ciliated microeukaryotes. Molecular Ecology Resources, 2023, 23(5): 1182-1193.
????6.Jing Zhang#, Weiwei Qin #, Che Hu#, Siyu Gu#, Xiaocui Chai, Mingkun Yang, Fang Zhou, Xueyan Wang, Kai Chen, Guanxiong Yan, Guangying Wang, Chuanqi Jiang, Alan Warren, Jie Xiong*, Wei Miao*. Giant proteins in a giant cell: molecular basis of ultrafast Ca2+-dependent cell contraction. Science Advances, 2023, 9(8): eadd6550.
????7.Mingyang?Ma, Chaojun?Wei, Wenjie?Huang, Yue?He, Yingchun?Gong*, Qiang?Hu*. A systematic review of the predatory contaminant?Poterioochromonas?in microalgal culture.?Journal of Applied Phycology, 2023, 35, 1103-1114.
????8.Wenya Song,?Jingyi Dong,?Xiaoteng Lu,?Saleh A Al-Farraj,?Weibo Song,?Hunter N Hines,?Xiaotian Luo*. Morphological, ontogenetic, and molecular investigations of freshwater hypotrich ciliates from China revealed a new genus Heterodeviata gen. nov. (Protista: Ciliophora), and a novel limnetic population of Deviata multilineae. Zoological Journal of the Linnean Society, 2023, 199, 263-279.
????9.Chengzhi Mao, Xiao Wang, Xinghao Li, Qiaoyi Kong, Elvis Genbo Xu*, Jie Huang*. Microbial communities, resistance genes, and resistome risks in urban lakes of different trophic states: internal links and external influences. Journal of Hazardous Materials Advances, 2023, 9, 100233.
????10.Chengzhi Mao#, Qi Li#, Majid Komijani, Jie Huang*, Tao Li*. Metagenomic analysis reveals the dissemination mechanisms and risks of resistance genes in plateau lakes. iScience, 2023, 26, 107508.
????11.Jiawei Tu, Tian Li, Zihan Gao, Jie Xiong, Wei Miao*. Construction of CdS-Tetrahymena thermophila hybrid system by efficient cadmium adsorption for dye removal under light irradiation. Journal of Hazardous Materials, 2022, 439, 129683.
????12.Hanwen Zhang,?David J Patterson,?Yue He,?Hongxia Wang,?Danni Yuan,?Qiang Hu,?Yingchun Gong*. Kinopus chlorellivorus gen. nov., sp. nov. (Vampyrellida, Rhizaria), a new algivorous protist predator isolated from large-scale outdoor cultures of Chlorella sorokiniana. Applied and Environmental Microbiology, 2022, 88(22): e0121522.
????13. 姜傳奇, 熊杰, 黃潔, 馮偉松, 龔迎春, 繆煒. 微型生物群落 eDNA-PFU 監(jiān)測.水生生物學(xué)報(bào), 2021 Nov 1;45(6).
????14.Mingyang Ma#,?Fuchen Wang#,?Chaojun Wei,?Jianping Chen,?Hu Jin,? Hongxia Wang,?Lirong Song,?Qiang Hu,?Yingchun Gong*. Establishment of high-cell-density heterotrophic cultivation of Poterioochromonas malhamensis contributes to achieving biological control of Microcystis. Journal of Applied Phycology, 2022, 34, 423-434.
????15.Pubo Chen#, Jie Huang#, Liuyu Rao, Wengen Zhu, Yuhe Yu, Fanshu Xiao*, Huang Yu, Yongjie Wu, Ruiwen Hu, Xingyu Liu, Zhili He, Qingyun Yan*. Environmental effects of nanoparticles on the ecological succession of gut microbiota across zebrafish development. 2022, Science of the Total Environment, 806, 150963.
????16.Xiaotian Luo,?William A Bourland,?Weibo Song,?Jie Huang*. New contributions to the taxonomy of urostylid ciliates (Ciliophora, Hypotrichia), with establishment of a new genus and new species. European Journal of Protistology, 2021, 80, 125810.
????17.Fanshu Xiao#, Wengen Zhu#, Yuhe Yu, Zhili He, Bo Wu, Cheng Wang, Longfei Shu, Xinghao Li, Huaqun Yin, Jianjun Wang, Philippe Juneau, Xiafei Zheng, Yongjie Wu, Juan Li, Xiaojuan Chen, Dongwei Hou, Zhijian Huang, Jianguo He, Guohuan Xu, Liwei Xie, Jie Huang*, Qingyun Yan*. Host development overwhelms environmental dispersal in governing the ecological succession of zebrafish gut microbiota. npj Biofilms and Microbiomes, 2021, 7, 5.
????18.Jie Xiong, Wentao Yang, Kai Chen, Chuanqi Jiang, Yang Ma, Xiaocui Chai, Guanxiong Yan, Guangying Wang, Dongxia Yuan, Yifan Liu, Shelby L. Bidwell, Nikhat Zafar, Michalis Hadjithomas, Vivek Krishnakumar, Robert S. Coyne, Eduardo Orias, Wei Miao*. Hidden genomic evolution in a morphospecies—The landscape of rapidly evolving genes in Tetrahymena. PLOS Biology, 2019, 17(6): e3000294.
????19.Xinghao Li,?Jie Huang*,?Sabine Filker,?Thorsten Stoeck,?Yonghong Bi*,?Yuhe Yu, Weibo Song. Spatio-temporal patterns of zooplankton in a main-stem dam affected tributary: a case study in the Xiangxi River of the Three Gorges Reservoir, China. Science China-Life Sciences, 2019, 62, 1058-1069.
????20.Miao Tian,?Wentao Yang,?Jing Zhang,?Huai Dang,?Xingyi Lu,?Chengjie Fu,?Wei Miao*. Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease. Nucleic Acids Research, 2017, 45(11): 6848-6863.
????21.Jie Xiong, Shan Gao, Wen Dui, Wentao Yang, Xiao Chen, Sean D. Taverna, Ronald E. Pearlman, Wendy Ashlock, Wei Miao* and Yifan Liu*. Dissecting relative contributions of cis- and trans-determinants to nucleosome distribution by comparing Tetrahymena macronuclear and micronuclear chromatin. Nucleic Acids Research, 2016, 44(21):10091-10105.
????22.韋薇, 袁冬霞, 楊文濤, 張晶, 陳瑛*, 繆煒*. 原生動(dòng)物柯立斯四膜蟲(Tetrahymena corlissi)超高砷耐受性的分子機(jī)制研究. 中國科學(xué):生命科學(xué), 2016, 46(03): 285-293.
????23.Marcella D Cervantes?,?Eileen P Hamilton,?Jie Xiong,?Michael J Lawson,?Dongxia Yuan,?Michalis Hadjithomas,?Wei Miao*,?Eduardo Orias*. Selecting one of several mating types through gene segment joining and deletion in Tetrahymena thermophila. PLOS Biology, 2013, 11(3): e1001518.
????24.Jie Xiong,?Yuming Lu,?Jinmei Feng,?Dongxia Yuan,?Miao Tian,?Yue Chang,?Chengjie Fu,?Guangying Wang,?Honghui Zeng*,?Wei Miao*. Tetrahymena functional genomics database (TetraFGD): an integrated resource for Tetrahymena functional genomics. Database, 2013, 2013, bat008.
