水生植物生理學(xué)研究組
研究組成員：?侯宏偉?研究員；houhw@ihb.ac.cn；
?????????????楊晶晶 副研究員,電子信箱：yangjj@ihb.ac.cn；
?????????????李高潔 助理研究員,電子信箱：ligaojie@ihb.ac.cn;?
?????????????李曉哲 助理實(shí)驗(yàn)師,電子信箱：lixiaozhe@ihb.ac.cn；
?????????????趙旭耀 特別研究助理,電子信箱：zhaoxuyao@ihb.ac.cn；
本研究組從事大型水生植物的研究與應(yīng)用。
1、水生植物生態(tài)、進(jìn)化與發(fā)育生物學(xué)研究。自然界生態(tài)因子的變化如何影響植物發(fā)育過程？植物發(fā)育過程如何響應(yīng)生態(tài)因子的變化？“植物-環(huán)境”相互作用與物種的適應(yīng)性進(jìn)化和生物多樣性之間存在緊密的聯(lián)系，物種的可塑性發(fā)育途徑對(duì)其生態(tài)適應(yīng)和進(jìn)化模式具有深遠(yuǎn)的影響。水生植物為適應(yīng)環(huán)境溫度、光照、水位等變化，不僅演化出適應(yīng)不同水陸生境的特征，還演化出了特有的水陸生境表型可塑性，本研究組在前期的探索中，發(fā)現(xiàn)一種爵床科的水族植物-異葉水蓑衣（Hygrophilla difformis）個(gè)體適中，異形葉特性明顯，是研究植物與環(huán)境的相互作用的理想材料（圖1），進(jìn)而從形態(tài)、生理、細(xì)胞和分子等方面研究其生物學(xué)特性及其對(duì)關(guān)鍵環(huán)境因子（溫度、光照、CO2、營(yíng)養(yǎng)等）的應(yīng)答策略，建立了研究模型，得以重新認(rèn)識(shí)和評(píng)價(jià)自然界生物遺傳和進(jìn)化的機(jī)制。此外，我們還研究浮萍、苦草、黃花貍藻等不同類群的水生植物，解析其淡水生態(tài)系統(tǒng)中水生植物的生物多樣性及其生態(tài)學(xué)意義（圖2）。
2、浮萍研究與應(yīng)用。浮萍是高等植物中一類個(gè)體小且簡(jiǎn)化、生長(zhǎng)快速的水生植物，是研究植物生理生化和生態(tài)毒理的理想材料，又由于其蛋白等含量高、營(yíng)養(yǎng)全面等特性，在開發(fā)替代蛋白食品、飼料和生物質(zhì)能等方面有巨大開發(fā)潛力（圖3）。本研究組依托國(guó)家水生生物資源庫(kù)，致力于浮萍種質(zhì)資源的收集和保藏，在此基礎(chǔ)上，開展不同浮萍的生物學(xué)特性、營(yíng)養(yǎng)和基因組研究和比較，挖掘功能基因，解析氮磷代謝和光合作用機(jī)制，建立遺傳轉(zhuǎn)化和高效培養(yǎng)的方法，創(chuàng)制高光合、高產(chǎn)、高蛋白浮萍種質(zhì)，優(yōu)化生產(chǎn)工藝，為建設(shè)浮萍垂直生產(chǎn)工廠、開發(fā)浮萍替代蛋白提供理論和技術(shù)支撐。
3、水生植物在生態(tài)、環(huán)境和生產(chǎn)上的應(yīng)用。本研究組對(duì)梁子湖等長(zhǎng)江流域的重要淡水湖泊開展水生植物調(diào)查，研究水生植物的分布、多樣性演變規(guī)律及其在淡水生態(tài)系統(tǒng)中的生態(tài)功能。篩選重要的水生經(jīng)濟(jì)植物品種（水芹等）進(jìn)行環(huán)境修復(fù)和生產(chǎn)應(yīng)用，建立利用水生經(jīng)濟(jì)植物聯(lián)合處理技術(shù)凈化沼液的循環(huán)利用模式（圖4），以及生態(tài)、高效的池塘魚-菜綜合種養(yǎng)及高效利用模式，并進(jìn)行示范推廣和應(yīng)用。
研究組代表論文：
1.??楊晶晶,趙旭耀,李高潔,胡詩(shī)琦,陳艷,孫作亮，侯宏偉*. 浮萍的研究及應(yīng)用進(jìn)展,科學(xué)通報(bào). 2021
2.??李高潔,胡詩(shī)琦,楊晶晶,侯宏偉*. 植物異形葉研究進(jìn)展. 植物生理學(xué)報(bào),2020
3.??吳志剛,熊文,侯宏偉*. 長(zhǎng)江流域水生植物多樣性格局與保護(hù). 水生生物學(xué)報(bào),2018
4.???Li GJ;Zhao XY;Yang JJ;Hu SQ;Ponnu J;Kimura S;Hwang I;Torii KU; Hou HW. Water wisteria genome reveals environmental adaptation and heterophylly regulation in amphibious plants. Plant Cell Environ. 2024
5.???Xia ML;Li XZ;Yang JJ;Li GJ;Zhao XY;Hou HW*. Cress-loach coculture for improving the utilization efficiency of biogas slurry in aquaponic systems. Environ Technol & Inno，2023
6.???Li YX;Xia ML;Zhao XY;Hou HW*. Water temperature and chlorophyll a density drive the genetic and epigenetic variation of Vallisneria natans across a subtropical freshwater lake. Ecol Evol. 2023.
7.???Li YX;Zhao XY;Xia ML;Wei XZ;Hou HW*. Temperature is a cryptic factor shaping the geographical pattern of genetic variation in Ceratophyllum demersum across a subtropical freshwater lake,Plant Diversity.2023.
8.???Sun ZL; Zhao XY; Li GJ; Yang JJ; Chen Y; Xia ML; Hwang I; Hou HW*, Metabolic flexibility during a trophic transition reveals the phenotypic plasticity of greater duckweed (Spirodela polyrhiza 7498), New Phytol. 2023,
9.???Li FI; Yang JJ; Sun ZY； Wang L； Qi LY；A S； Liu YQ； Zhang HM； Dang LF； Wang SJ； Luo CX； Nian WF； O'Conner S； Ju LZ； Quan WP； Li XK； Wang C； Wang DP； You HL； Cheng ZK； Yan J； Tang FC； Yang DC； Xia CW； Gao G； Wang Y； Zhang BC； Zhou YH； Guo X； Xiang SH； Liu H； Peng TB； Su XD； Chen Y； Ouyang Q； Wang DH； Zhang DM； Xu ZH； Hou HW； Bai SN； Li L. Plant-on-chip: Core morphogenesis processes in the tiny plant Wolffia australiana. PNAS Nexus. 2023
10.?? Ikematsu S； Umase T; Shiozaki M; Nakayama S; Noguchi F; Sakamoto T; Hou HW; Gohari G; Kimura S; Torii KU. Rewiring of hormones and light response pathways underlies the inhibition of stomatal development in an amphibious plant Rorippa aquatica underwater. Curr Biol. 2023
11.?? Li GJ; Yang, JJ; Chen YM; Zhao, XY; Chen Y; Kimura S; Hu SQ; Hou,HW*, SHOOT MERISTEMLESS participates in the heterophylly of Hygrophila difformis (Acanthaceae). Plant Physiol, 2022
12.?? Sun ZL; Zhao XY; Xia ML; Yang JJ; Chen Y; Li XZ; Hou,HW*, Use of hemicellulose-derived xylose for environmentally sustainable starch production by mixotrophic duckweed. Sustain Energ Fuels, 2022
13.?? Zhao XY; Yang JJ; Li XZ; Li GJ; Sun ZL; Chen Y; Chen YM; Xia ML; Li YX; Yao LG; Hou HW*, Identification and expression analysis of GARP superfamily genes in response to nitrogen and phosphorus stress in Spirodela polyrhiza. BMC Plant Biol, 2022
14.?? Sun ZL; Guo WJ; Zhao XY; Chen Y; Yang JJ; Xu SQ; Hou HW*, Sulfur limitation boosts more starch accumulation than nitrogen or phosphorus limitation in duckweed (Spirodela polyrhiza). Ind Crop Prod, 2022
15.?? Yang JJ; Li GJ; Xia ML; Chen YM; Chen Y; Kumar S; Sun ZL; Li XZ; Zhao XY*; Hou HW*,? Combined effects of temperature and nutrients on the toxicity of cadmium in duckweed (Lemna aequinoctialis). J Hazard Mater, 2022
16.?? Yang JJ; Zhao XY; Chen Y; Li GJ; Li XZ; Xia ML; Sun ZL; Chen YM; Li YX; Yao LG; Hou HW*, Identification, structural, and expression analyses of SPX genes in giant duckweed (Spirodela polyrhiza) reveals its role in response to low phosphorus and nitrogen Stresses. Cells, 2022
17.?? Chen Y; Li GJ; Yang JJ; Zhao XY; Sun ZL; Hou HW*, Role of nramp transporter genes of Spirodela polyrhiza in cadmium accumulation. Ecotox Environ Safe, 2021
18.?? Yang JJ; Zhao XY; Li GJ; Hu SQ; Hou HW*, Frond architecture of the rootless duckweed Wolffia globosa. BMC Plant Biol, 2021
19.?? Kumar S; Li GJ; Yang JJ; Huang XF; Ji Q; Liu ZW; Ke WD*; Hou HW*, Effect of salt stress on growth, physiological parameters, and ionic concentration of water dropwort (Oenanthe javanica) cultivars. Front Plant Sci. 2021
20.?? Zhao XY; Li GJ; Sun ZL; Chen Y; Guo WJ; Li YX; Chen YM; Yang JJ*; Hou HW*, Identification, structure analysis, and transcript profiling of phosphate transporters under Pi deficiency in duckweeds. Int J Biol Macromol. 2021
21.?? Zhao XY; Yang JJ; Li GJ; Sun ZL; Hu SQ; Chen Y; Guo WJ; Hou HW*, Genome-wide identification and comparative analysis of the WRKY gene family in aquatic plants and their response to abiotic stresses in giant duckweed (Spirodela polyrhiza). Genomics. 2021
22.?? Kumar S; Li, GJ; Yang JJ; Huang XF; Ji Q; Zhou K; Khan? S; Ke WD*; Hou HW*, Investigation of an antioxidative system for salinity tolerance in Oenanthe javanica. Antioxidants. 2020
23.?? Sun ZL; ?Guo WJ; ?Yang JJ; ?Zhao XY; Chen Y; Yao LG; Hou HW*. Enhanced biomass production and pollutant removal by duckweed in mixotrophic conditions. Bioresour Technol. 2020
24.?? Heenatigala PPM; Sun ZL; Yang JJ; Zhao XY; Hou HW*, Expression of LamB vaccine antigen in Wolffia globosa (Duckweed) against fish vibriosis. Front Immunol. 2020
25.?? Li GJ #; ?Hu SQ #; ?Yang JJ; ?Zhao XY; ?Schultz E; ?Kimura S; Hou HW*, Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae), Plant Cell Rep. 2020
26.?? Wu ZG; Xu X; Zhang J; Wiegleb G; Hou HW*, Influence of environmental factors on the genetic variation of the aquatic macrophyte Ranunculus subrigidus on the Qinghai-Tibetan Plateau. BMC Evol Biol. 2019
27.?? Yang JJ; Li GJ; Hu SQ; Bishopp A; Heenatigala PPM; Kumar S; Duan PF; Yao LG; Hou HW*, A protocol for efficient callus induction and stable transformation of Spirodela polyrhiza (L.) Schleiden using Agrobacterium tumefaciens. Aquat Bot. 2018
28.?? Mariyamma NP; Hou HW; Carland FM; Nelson T; Schultz EA*, Localization of Arabidopsis FORKED1 to a RABA positive compartment suggests a role in secretion. J Exp Bot. 2017
29.?? Mariyamma N; Clarke K; Yu H; Hou HW; Wilton E; Dyk J; Schultz E*, Members of the Arabidopsis FORKED1-LIKE gene family act to localize PIN1 in developing veins. J Exp Bot. 2018
30.?? Li GJ; Hu SQ; Yang JJ; Schultz E; Clarke K; Hou HW*, Water-Wisteria as an ideal plant for heterophylly study in higher aquatic plants. Plant Cell Rep. 2017
31.?? Wu Z; Wu J; Wang Y; Hou HW*, Development of EST-derived microsatellite markers in the aquatic macrophyte Ranunculus bungei (Ranunculaceae). Applications in Plant Sciences. 2017
32.?? Mariyamma N; Hou HW; Carland F; Nelson T; Schultz E*, Localization of Arabidopsis FORKED1 to a RABA positive compartment suggests a role in secretion. J Exp Bot. 2017
33.?? Khan S; Nabi G; Ullah MW; Yousaf M; Manan S; Siddique R; Hou HW*, Overview on the role of advance genomics in conservation biology of endangered species. Int J Genomics. 2016
34.?? Held M#; Hou HW#; Miri M#; Huynh C; Ross L; Hossain S; Sato S; Tabata S; Perry J; Wang T; Szczyglowski K*, Lotus japonicus cytokinin receptors work partially redundantly to mediate nodule formation. Plant Cell. 2014
35.?? Yoon H#; Hossain M#; Held M; Hou HW; Kehl M; Tromas A; Sato S; Tabata T; Andersen S; Stougaard J; Ross L; Szczyglowski K*, Lotus japonicus SUNERGOS1 encodes apredicted subunit A of a DNA topoisomerase VI and is required for nodule differentiationand accommodation of rhizobial infection. Plant J. 2014
36.?? Garrett J#; Meents M; Blackshaw M; Blackshaw L; Hou HW; Styranko D; Kohalmi S; Schultz E*, A novel, semi-dominant allele of MONOPTEROS provides insight into leaf initiation and vein pattern formation. Planta. 2012
37.?? Hou HW; Erickson J; Meservy J; Schultz E*, FORKED1 encodes a PH domain proteinthat is required for PIN1 canalization in developing leaf veins. Plant J. 2010
38.?? Hou HW; Zhou Y; He X; Mwange K; Li W; Cui K*, The ABP1 expression regulated by IAA and ABA is associated with the cambial activity periodicity in Eucommia ulmoides. J Exp Bot. 2006
39.?? Mwange, K#; Hou, HW#;Wang, Y; He, X; Cui, K*, Opposite patterns in the annual distribution and time course of endogenous abscisic acid and indole-3-acetic acid in relation to the periodicity of cambial activity in Eucommia ulmoides. J Exp Bot. 2005
40.?? Mwange, K; Hou, HW; Cui, K*, Relationship between endogenous indole-3-acetic acid and abscisic acid changes and bark recovery in Eucommia ulmoides after girdling. J Exp Bot. 2003
