藍(lán)藻生長發(fā)育與合成生物學(xué)
?????? 學(xué)科負(fù)責(zé)人：張承才?研究員? ?? ? ?
學(xué)科組成員：?? ?
????? 青年研究員：曾曉麗
????? 副研究員：楊毅玲、張巨源、Cesar Augusto VALADES CRUZ、邢偉越?
實驗師：何晨柳?
科研助理：林桂銘、余昭君、湯運(yùn)斌?
博士后：徐曉梅、孫慶學(xué)、劉靜、劉素娟?
博士生：李文凱、冉文碩、李尚諭、曹淑可、關(guān)文盈、于帆?
碩士生：付婕、黃丹青、解濤、田霞霞、賈世豪、喻言、王振宇、李佩蓮、孟令涵?
招生導(dǎo)師及招生方向：?
博 導(dǎo)：張承才（碩士、博士：遺傳學(xué)）?
????? 碩 導(dǎo)：楊毅玲（碩士：遺傳學(xué)），張巨源（碩士：遺傳學(xué)），曾曉麗（碩士：遺傳學(xué)）
學(xué)科組介紹：
??????學(xué)者張承才于2015年加入水生所并成立藻類生長與發(fā)育學(xué)科組。本學(xué)科組以藍(lán)藻作為材料，以分子遺傳學(xué)和細(xì)胞生物學(xué)為手段研究細(xì)胞生長與發(fā)育的機(jī)制，包括細(xì)胞發(fā)育和細(xì)胞周期的關(guān)系，及對環(huán)境變化適應(yīng)能力分子機(jī)制。?
藍(lán)藻出現(xiàn)在二十五億年前，是地球上最古老的生命之一。它們是最早能進(jìn)行放氧光合作用的生物，其產(chǎn)生的氧氣使地球大氣層由無氧轉(zhuǎn)換為富氧，進(jìn)而為整個地球環(huán)境動植物和人類的進(jìn)化打下了基礎(chǔ)。藍(lán)藻廣泛存在于海洋、湖泊、濕地、荒漠等各種生態(tài)環(huán)境中，目前依然為地球碳氮氧等元素循環(huán)起著巨大的作用。?
近年來，藍(lán)藻日益受到人們關(guān)注。一方面，在富營養(yǎng)化水體中，藍(lán)細(xì)菌經(jīng)常呈暴發(fā)性生長（形成水華），造成極大的環(huán)境問題。另一方面，雖然藍(lán)藻能利用太陽能將CO2轉(zhuǎn)化為油脂、藥物等化合物，在綠色能源及制藥等領(lǐng)域顯示出巨大應(yīng)用潛力，但其與大多工業(yè)微生物相比生長速率相對緩慢，這在很大程度上制約了對其規(guī)模應(yīng)用。因此，無論治理藍(lán)藻水華，還是對藍(lán)藻進(jìn)行開發(fā)利用，其核心都涉及對其生物量的控制。本學(xué)科組致力于對藍(lán)藻生長過程及其調(diào)控機(jī)理進(jìn)行研究，以期能為抑制水華爆發(fā)及構(gòu)建高效藍(lán)藻生物反應(yīng)器提供理論依據(jù)。?? ?
藍(lán)藻也是用于研究許多重要基礎(chǔ)生物學(xué)問題的模式生物。不同藍(lán)藻的細(xì)胞大小各異、形態(tài)多樣；部分藍(lán)藻還具有細(xì)胞發(fā)育的能力，例如形成執(zhí)行固氮功能的異形胞（最古老的細(xì)胞分化現(xiàn)象之一）。細(xì)胞大小、形態(tài)以及細(xì)胞發(fā)育通過怎樣的分子機(jī)制進(jìn)行調(diào)控？它們同代謝調(diào)控、細(xì)胞生長、細(xì)胞周期等生物過程的關(guān)系又是如何確立的？本學(xué)科組以藍(lán)藻為材料對這些重大科學(xué)理論問題開展深入研究。?
我們的研究手段強(qiáng)調(diào)多學(xué)科融合，涉及光學(xué)及實驗物理學(xué)技術(shù)，單細(xì)胞操作技術(shù)，組學(xué)技術(shù)，生理、遺傳、生化與分子生物學(xué)技術(shù)等。學(xué)科組在藍(lán)藻分子遺傳學(xué)特別是藍(lán)藻異形胞發(fā)育研究方面已取得國際公認(rèn)的學(xué)術(shù)成就，在國際期刊上發(fā)表文章90余篇，其中包括PNAS, Mol Microbiol, Nucl Acid Res, J Bacteriol. , FEMS Microbiol Rev等雜志。 此外，藍(lán)藻在特定生長階段會向環(huán)境中釋放毒素及其他一些具有重要生態(tài)效應(yīng)的次級代謝產(chǎn)物，本學(xué)科組也致力于闡明這些代謝產(chǎn)物在胞內(nèi)的合成及代謝途徑。?
儀器設(shè)備：?
熒光顯微鏡；生物顯微鏡；微流控操作平臺；細(xì)胞破碎儀；PCR儀；超微分光光度計；核酸、蛋白分離檢測系統(tǒng)；蛋白純化系統(tǒng)；組學(xué)分析儀器；紫外分光光度計；冷凍高速離心機(jī)；微藻高通量培養(yǎng)系統(tǒng)；低溫超高壓連續(xù)流細(xì)胞破碎儀；制冰機(jī)；氮吹儀；光照培養(yǎng)箱等。?
在研主持項目（部分）：?
????國家重點研發(fā)計劃合成生物學(xué)重點專項?
國家自然科學(xué)基金重大研究計劃?
國家自然科學(xué)基金重點項目?
國家自然科學(xué)基金面上項目?
國家自然科學(xué)基金青年基金?
近年發(fā)表論文：?
2024年度?
1） Su-Juan Liu, Gui-Ming Lin, Yu-Qi Yuan, Wenli Chen, Ju-Yuan Zhang* and Cheng-Cai Zhang* (2024) A protein inhibitor brings under check the activity of RNase E in cyanobacteria.?Nucl. Acids Res. 52:404-419.?
2） Ye-Jun Peng, Yuxing Chen, Cong-Zhao Zhou, Wei Miao, Yong-Liang Jiang*, Xiaoli Zeng* and Cheng-Cai Zhang* (2024) Modular catalytic activity of nonribosomal peptide synthetases depends on the dynamic interaction between adenylation and condensation domains.?Structure. 32(1-13):e1-e4?
2023年度?
1)?Qin-Xue Sun, Min Huang, Ju-Yuan Zhang, Xiaoli Zeng*, Cheng-Cai Zhang* (2023) Control of Cell Size by c-di-GMP Requires a Two-Component Signaling System in the Cyanobacterium Anabaena sp. Strain PCC 7120.?Microbiology Spectrum. 10.1128?
2)?Xiaoli Zeng, Min Huang, Qing-Xue Sun, Ye-Jun Peng, Xiaomei Xu, Yun-Bin Tang, Ju-Yuan Zhang, Yiling Yang, Cheng-Cai Zhang* (2023) A c-di-GMP binding effector controls cell size in a cyanobacterium.?PNAS. 120(13):e2221874120?
3)?Zi-Qian Wang, Yiling Yang, Ju-Yuan Zhang, Xiaoli Zeng, Cheng-Cai Zhang* (2023) Global translational control by the transcriptional repressor TrcR in the filamentous cyanobacterium Anabaena sp. PCC 7120.?Communications Biology.?6:643?
4)?Wei-Yue Xing, Jing Liu, Cheng-Cai Zhang* (2023) HetF defines a transition point from commitment to morphogenesis during heterocyst differentiation in the cyanobacterium Anabaena sp. PCC 7120.?Molecular Microbiology. 00:740-753.?
5)?Jing Liu, Wei-Yue Xing, Bowen Liu, Cheng-Cai Zhang* (2023) Three-dimensional coordination of cell-division site positioning in a filamentous cyanobacterium.?PNAS nexus. 10.1093?
6)?Ziqian Wang, Suqin Wang, Ju-Yuan Zhang, Gui-Ming Lin, Nanqin Gan, Lirong Song, Xiaoli Zeng, Cheng-Cai Zhang* (2023) Investigation on cyanobacterial production of the proposed neurotoxin??-N-methylamino-L-alanine (BMAA).?Water Biol. Security. P 100208.?
2022年度?
1)??Wei-Yue Xing, Jing Liu, Ju-Yuan Zhang, Xiaoli Zeng, Cheng-Cai Zhang* (2022) A proteolytic pathway coordinates cell division and heterocyst differentiation in the cyanobacterium Anabaena sp. PCC 7120.?PNAS. 119 (36):e2207963119?
2)??Xiaoli Zeng*,?Cheng-Cai Zhang* (2022) The making of a heterocyst in cyanobacteria.?Annu Rev Microbiol.?76:597-618.?
3)??JuYuan Zhang, Wolfgang R. Hess*, Cheng-Cai Zhang* (2022) “Life is short, and art is long”: RNA degradation in cyanobacteria and model bacteria.?mLife. 00(00):1-19?
4)??Zi-Qian Wang, Cheng-Cai Zhang* (2022) A tRNA t6A modification system contributes to the sensitivity towards the toxin -N-methylamino-L-alanine (BMAA) in the cyanobacterium Anabaena sp. PCC 7120.?Aquatic Toxicology. 245:106121?
5)??Ju-Yuan Zhang, Tian-Cai Niu, Gui-Ming Lin, Cheng-Cai Zhang (2022) A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria.?Essential Genes and Genomes. Part of the Methods in Molecular Biology book series (MIMB, volume 2377). pp 143-157.?
2021年度?
1)??Wei-Yue Xing, Jing Liu, Zi-Qian Wang, Ju-Yuan Zhang, Xiaoli Zeng, Yiling Yang and Cheng-Cai Zhang* (2021) HetF protein is a new divisome component in a filamentous and developmental cyanobacterium.?mBio. 12:e01382-21. ?
2)??Li Wang, Tian-Cai Niu, Ana Valladares, Gui-Ming Lin, Ju-Yuan Zhang, Antonia Herrero*, Wenli Chen*, Cheng-Cai Zhang* (2021) The developmental regulator PatD modulates assembly of the cell-division protein FtsZ in the cyanobacterium Anabaena sp. PCC 7120.?Environmental Microbiology. 23(8):4823-4837.?
3)??Jing Liu, Wei-Yue Xing, Ju-Yuan Zhang, Xiaoli Zeng, Yiling Yang and Cheng-Cai Zhang* (2021) Functions of the Essential GenemraY in Cellular Morphogenesis and Development of the Filamentous Cyanobacterium Anabaena PCC 7120.?Frontiers in Microbiology.?12:765878.?
4)? Min Huang, Ju-Yuan Zhang, Xiaoli Zeng* and Cheng-Cai Zhang (2021) c-di-GMP Homeostasis Is Critical for Heterocyst Development in Anabaena sp. PCC 7120.?Frontiers in Microbiology. 12:793336??
2020年度?
1)??Zi-Qian Wang, Suqin Wang, Ju-Yuan Zhang, Gui-Ming Lin, Nanqin Gan , Lirong Song, Xiaoli Zeng* & Cheng-Cai Zhang* (2020) The proposed neurotoxin??-n-methylamino-l-alanine (bmaa) is taken up through amino-acid transport systems in the cyanobacterium anabaena pcc 7120.?Toxins.?12(8):518. ?
2)??Xing, W. Y. , Xie, L. R. , Zeng, X. , Yang, Y. *, & Zhang, C. C. * (2020) Functional dissection of genes encoding dna polymerases based on conditional mutants in the heterocyst-forming cyanobacterium anabaena pcc 7120.?Frontiers in Microbiology.?11:1108.?
3)??Cong Zhou, Juyuan Zhang , Xinyu Hu, Changchang Li , Li Wang , Qiaoyun Huang* and Wenli Chen* (2020) Rnase ii binds to rnase e and modulates its endoribonucleolytic activity in the cyanobacterium anabaena pcc 7120.?Nucleic Acids Research. 48(7):3922-3934?
2019年度?
1)??Li Wang, Tian-Cai Niu, Gui-Ming Lin, Shao-Ran Zhang, Ju-Yuan Zhang, Guo-Fang Tang, Wenli Chen*, Cheng-Cai Zhang* (2019) patD, a gene regulated by NtcA, is involved in the optimization of heterocyst frequency in the cyanobacterium Anabaena PCC 7120.?J. Bacteriol.?201(21). pii:e00457-19.? ?
2)??Wei-Yue Xing, Cheng-Cai Zhang* (2019) Preventing accidental heterocyst development in cyanobacteria.?J. Bacteriol. 201 (17):e00349-19. ?
3)??Tian-Cai Niu, Gui-Ming Lin, Li-Rui Xie, Zi-Qian Wang, Wei-Yue Xing, Ju-Yuan Zhang*, Cheng-Cai Zhang (2019) Expanding the potential of CRISPR-Cpf1 based genome editing technology in the cyanobacterium Anabaena PCC 7120.?ACS Synth. Biol.?8:170-180.?
2018年度?
1)? Cheng-Cai Zhang*, Cong-Zhao Zhou, Robert L. Burnap, Ling Peng (2018) Carbon/Nitrogen Metabolic Balance: Lessons from Cyanobacteria.?Trends in Plant Sci.?23:1116-1130.
2)??Hai-Lin Chen, Amel Latifi, Cheng-Cai Zhang and Christophe S bastien Bernard* (2018) Biosensors-Based In Vivo Quantification of 2-Oxoglutarate in Cyanobacteria and Proteobacteria.?Life. 8:51. ?
3)??Yali Wang, Yuan Gao, Chao Li, Hong Gao, Cheng-Cai Zhang and Xudong Xu* (2018) Three substrains of the cyanobacterium Anabaena sp. PCC 7120 display divergence in genomic sequences and hetC function.?J. Bacteriol. 200 (13):e00076-18. ?
4)??Ju-Yuan Zhang, Gui-Min Lin, Wei-Yue Xin, Cheng-Cai Zhang* (2018) Diversity of the growth patterns probed in live cyanobacterial cells using a fluorescent analog of a peptidoglycan precursor.?Frontiers in Microbiology.?9:791. ?
5)? Yong-Liang Jiang, Xue-Ping Wang, Hui Sun, Shu-Jing Han, Wei-Fang Li, Ning Cui, Gui-Ming Lin, Ju-Yuan Zhang, Wang Cheng, Dong-Dong Cao, Zhi-Yong Zhang, Cheng-Cai Zhang*, Yuxing Chen*, Cong-Zhao Zhou* (2018) Coordinating carbon and nitrogen metabolic signaling through the cyanobacterial global repressor NdhR.?PNAS. 115:403-408.??? ??
2017年度?
1)??Cheng, Y., Schorey, JS., Zhang, C-C., and Tan X. (2017) Protein kinase inhibitors as potential antimicrobial drugs against Tuberculosis, Malaria and HIV.?Curr. Pharm. Des.?23:4369-4389.? ??
2015年度?
1)? Hu, S.; Wang, J.; Wang, L.; Zhang, C.-C.; Chen, W. (2015) Dynamics and cell-type specificity of the DNA double-strand break repair protein RecN in the developmental cyanobacterium Anabaena sp. strain PCC 7120.?PLoS One.10(10):e0139362. ?
2)? Fan Y, Lemeille, S., Gonz lez, A., Risoul, V., Denis, Y., Richaud, P., Lamrabet, O., Fillat, M.,?Zhang, C.-C., Latifi A. (2015) The Pkn22 Ser/Thr kinase in Nostoc PCC 7120: role of FurA and NtcA regulators and transcript profiling under nitrogen starvation and oxidative stress.?BMC Genomics. 16:557.?
3)??Hu, H-X., Jiang, Y-L., Zhao, M-X., Cai K., Liu, S., Wen, B., Lv, P., Zhang, Y., Peng, J., Yu, H.-M., Ren, Y.-M., Zhang, Z., Wu, Q., Oliveberg, M., Zhang, C.-C*., Chen, Y*., Zhou, C.Z.* (2015) Structural insights into HetR PatS interaction involved in cyanobacterial pattern formation.?Sci. Rep.?5:16470.?
