硅藻與生物合成研究組

學(xué)科帶頭人:胡晗華?研究員?
學(xué)科組成員:潘玉芳高級(jí)實(shí)驗(yàn)師、金虎項(xiàng)目高級(jí)工程師、博士后2人、博士研究生2人、碩士研究生4人。?
學(xué)科組介紹:硅藻是地球上最重要的初級(jí)生產(chǎn)者之一,貢獻(xiàn)了全球近20%的碳固定。本研究組成立于2011年,主要以硅藻為對(duì)象,理論方面主要研究其脂質(zhì)代謝、氮代謝和光合碳固定的機(jī)制,應(yīng)用上利用合成生物學(xué)等手段,創(chuàng)制具有高效光合性能和抗逆能力的硅藻底盤細(xì)胞,同時(shí)結(jié)合微藻發(fā)酵技術(shù),發(fā)展硅藻生物制造的相關(guān)技術(shù)。?
代表性論文(*示通訊作者):?
硅藻
[1].Ge F,Huang W,Chen Z,Zhang C,Xiong Q,Bowler C,Yang J,Xu J,Hu H*. 2014. Methylcrotonyl-CoA carboxylase regulates triacylglycerol accumulation in the model diatom Phaeodactylum tricornutum. The Plant Cell. 26(4):1681–1697.
[2].Pan Y,Zhang W,Wang X,Jouhet J,Maréchal E,Liu J,Xia X,Hu H*. 2024. Allele-dependent expression and functionality of lipid enzyme phospholipid:diacylglycerol acyltransferase affect diatom carbon storage and growth. Plant Physiology. 194(2):1024–1040.
[3].Huang T,Pan Y,Maréchal E,Hu H*. 2024. Proteomes reveal the lipid metabolic network in the complex plastid of Phaeodactylum tricornutum. The Plant Journal. 117(2):385–403.
[4].Pan Y,Yang J,Gong Y,Li X,Hu H*. 2017. 3-hydroxyisobutyryl-CoA hydrolase involved in isoleucine catabolism regulates triacylglycerol accumulation in Phaeodactylum tricornutum. Philosophical Transactions of the Royal Society B‐Biological Sciences. 372(1728):20160409.
[5].Rastogi A,Vieira FRJ,Deton-Cabanillas A-F,Veluchamy A,Cantrel C,Wang G,Vanormelingen P,Bowler C,Piganeau G,Hu H*,Tirichine L*. 2020. A genomics approach reveals the genetic polymorphism,structure and functional diversity of ten accessions of the diatom Phaeodactylum tricornutum. The ISME Journal. 14: 347–363.
[6].Schoefs B*,Hu H*,Kroth PG*. 2017. The peculiar carbon metabolism in diatoms. Philosophical Transactions of the Royal Society B‐Biological Sciences. 372(1728):20160405.
[7].Allen AE,Dupont CL,Oborník M,Horák A,Nunes-Nesi A,McCrow JP,Zheng H,Johnson DA,Hu H,Fernie AR,Bowler C. 2011. Evolution and metabolic significance of the urea cycle in photosynthetic diatoms. Nature. 473(7346):203–207.
[8].You L,Po?ońska A,Jasieniecka-Gazarkiewicz K,Richard F,Jouhet J,Maréchal E,Bana? A,Hu H,Pan Y,Hao X,Jin H,Allen AE,Amato A,Gong Y. 2024. Two plastidial lysophosphatidic acid acyltransferases differentially mediate the biosynthesis of membrane lipids and triacylglycerols in Phaeodactylum tricornutum. New Phytologist. 241(4):1543–1558.
[9].Seydoux C,Storti M,Giovagnetti V,Matuszyńska A,Guglielmino E,Zhao X,Giustini C,Pan Y,Blommaert L,Angulo J,Ruban AV,Hu H,Bailleul B,Courtois F,Allorent G,Finazzi G. 2022. Impaired photoprotection in Phaeodactylum tricornutum KEA3 mutants reveals the proton regulatory circuit of diatoms light acclimation. New Phytologist. 234(2):578?591.
[10]. Hao X,Chen W,Amato A,Jouhet J,Maréchal E,Moog D,Hu H,Jin H,You L,Huang F,Moosburner M,Allen AE,Gong Y. 2022. Multiplex CRISPR/Cas9 editing of the long-chain acyl-CoA synthetase family in the diatom Phaeodactylum tricornutum reveals that mitochondrial ptACSL3 is involved in the synthesis of storage lipids. New Phytologist. 233(4):1797–1812.
微擬球藻
[11]. Yang J,Liu J,Pan Y,Maréchal E,Amato A,Liu M,Gong Y,Li Y,Hu H*. PDAT regulates PE as transient carbon sink alternative to triacylglycerol in Nannochloropsis. 2022. Plant Physiology. 189(3):1345?1362.
[12]. Hu H*,Gao K. 2006. Response of growth and fatty acid compositions of Nannochloropsis sp. to environmental factors under elevated CO2 concentration. Biotechnology Letters. 28(13):987?992.
[13]. Hu H,Gao K. 2003. Optimization of growth and fatty acid composition of a unicellular marine picoplankton,Nannochloropsis sp.,with enriched carbon sources. Biotechnology Letters. 25(5):421?425.
[14]. Xin Y,Lu Y,Lee Y-Y,Wei L,Jia J,Wang Q,Wang D,Bai F,Hu H,Hu Q,Liu J,Li Y,Xu J. 2017. Producing designer oils in industrial microalgae by rational modulation of co-evolving type-2 diacylglycerol acyltransferases. Molecular Plant. 10(12):1523?1539.
方法學(xué)
[15]. Yin W,Hu H*. 2023. CRISPR/Cas9-mediated genome editing via homologous recombination in a centric diatom Chaetoceros muelleri. ACS Synthetic Biology. 12(4):1287?1296.
[16]. Yin W,Hu H*. 2021. High-efficiency transformation of a centric diatom Chaetoceros muelleri by electroporation with a variety of selectable markers. Algal Research. 55: 102274.
[17]. Chen Y,Hu H*. 2019. High efficiency transformation by electroporation of the freshwater alga Nannochloropsis limnetica. World Journal of Microbiology & Biotechnology. 35(8):119.
[18]. Wei L,Xin Y,Wang Q,Yang J,Hu H*,Xu J*. 2017. RNAi-based?targeted?gene-knockdown?in?the?model?oleaginous?microalgae?Nannochloropsis?oceanica. The Plant Journal. 89(6):1236?1250.
[19]. Zhang C,Hu H*. 2014. High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation. Marine Genomics. 16: 63?66.
[20]. Li F,Gao D*,Hu H*. 2014. High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product. Bioscience Biotechnology and Biochemistry. 78(5):812?817.
